SARS-CoV-2 (2019-nCoV) Nucleoprotein/NP ELISA Kit
This SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of 2019-nCoV CoV Nucleocapsid protein in Serum or Cell supernatant. It contains recombinant 2019-nCoV CoV Nucleocapsid, and antibodies raised against the recombinant protein.
This ELISA kit is complete and ready-to-use.
Product Name: SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ELISA Kit
Assay Type: Solid Phase Sandwich ELISA (quantitative)
Sample Type: Serum, Cell supernatant
Sensitivity: 42.5 pg/ml
Assay Range: 93.75-6000 pg/ml
Specificity: Recognizes both recombinant and natural 2019-nCoV CoV Nucleocapsid
– 96 well microplate coated with Capture Antibody
– Detection Antibody conjugated to HRP
– Wash Buffer Concentrate
– Dilution Buffer Concentrate
– Color Reagent A
– Color Reagent B
– Stop Solution
Unopened Kit: Store at +2 – 8°C
Opened/Reconstituted Reagents: Please refer to CoA.
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.