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Forward Primer for SARS-CoV-2 detection (50 qPCR reactions)

20µL

Product: BCC-19nCoVN2_F50

BaseClick provides primers and probes recommended by the Centers for Disease Control and Prevention to researchers for the in vitro detection of the novel coronavirus (2019-nCoV). The primers and probes are designed for the universal detection of SARS-like coronaviruses (N3 assay) and for the specific detection of novel 2019-nCoV (N1 and N2 assays).
RP – all clinical samples should be tested for human RNAse P (RNP) gene to assess specimen quality.
If you are interested in primers and probes to create a Real-Time RT-PCR panel in your lab, follow the panel instructions from the CDC to get more instructions for use.

Catalog No.DescriptionSequence (5’-3’)LengthLabel
BCC-19nCoVN2_F50Forward Primer for 50 qPCR reactionsTTA CAA ACA TTG GCC GCA AA20-
BCC-19nCoVN2_R50Reverse Primer for 50 qPCR reactionsGCG CGA CAT TCC GAA GAA18-
BCC-19nCoVN2_P50Probe for 50 qPCR reactionsFAM-ACA ATT TGC CCC CAG CGC TTC AG -BHQ123FAM / BHQ1
BCC-19nCoVN2_S50Set of N2 Primers & Probe for 50 qPCR reactionsTTA CAA ACA TTG GCC GCA AA
GCG CGA CAT TCC GAA GAA
FAM-ACA ATT TGC CCC CAG CGC TTC AG -BHQ1
20
18
23
-
-
FAM / BHQ1
BCC-19nCoVN2_F100Forward Primer for 100 qPCR reactionsTTA CAA ACA TTG GCC GCA AA20-
BCC-19nCoVN2_R100Reverse Primer for 100 qPCR reactionsCG CGA CAT TCC GAA GAA18-
BCC-19nCoVN2_P100Probe for 100 qPCR reactionsFAM-ACA ATT TGC CCC CAG CGC TTC AG -BHQ123FAM / BHQ1
BCC-19nCoVN2_S100Set of N2 Primers & Probe for 100 qPCR reactionsTTA CAA ACA TTG GCC GCA AA
GCG CGA CAT TCC GAA GAA
FAM-ACA ATT TGC CCC CAG CGC TTC AG -BHQ1
20
18
23
-
-
FAM / BHQ1

These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations.

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