CleanMag Magnetic Beads 1 mL (9 reactions)

£55.00

1 mL

Product Code: 718001 Category: Tags: , ,

CleanMag Magnetic Beads for high-throughput purification of PCR amplicons remove excess primers, nucleotides, salts and enzymes for purer PCR product.

High Recovery of DNA after when stored with CleanMag Magnetic Beads
CleanMag Magnetic Beads were used to purify DNA ladder at various bead-to-sample volume ratios, and the recovered yields at different size ranges were quantitated using an Agilent Bioanalyzer and compared with the starting input amounts to determine recovery. The results demonstrate that CleanMag Magnetic Beads minimize loss of even short DNA fragments after purification.
High Recovery of DNA after when stored with CleanMag Magnetic Beads
CleanMag Magnetic Beads were used together with a CleanPlex NGS Panel to construct targeted NGS libraries. The resulting purified libraries were stored with CleanMag Magnetic Beads for 8 days at 2-8 C, and the recovered yields at different size ranges were quantitated using an Agilent Bioanalyzer and compared with those prior to storage to determine recovery.

Product Description

The CleanMag Magnetic Beads offer a highly consistent solution for reaction purification and are developed for use in targeted library construction using CleanPlex® and CleanPlex UMI NGS Panels. CleanMag Magnetic Beads are formulated in a buffered solution to quickly bind PCR amplicons and other DNA products above 100 bp, allowing for the removal of excess primers, nucleotides, salts, and enzymes. The reversible nature of the binding interaction between beads and PCR amplicons enables easy elution and separation following purification.

Volume: 1 mL of Magnetic beads (~9 CleanPlex 1-pool panel reactions).
Weight:35 g
Dimensions: 11x11x10 cm
Highlights

  • Seamless Integration into CleanPlex Workflows
  • Compatible and validated with all CleanPlex and CleanPlex UMI targeted library preparation protocols
  • Versatile
  • Compatible with a variety of DNA and RNA NGS library preparation chemistries, as well as manual and automated processing.
  • High Performance
  • Fast, consistent, and efficient purification with high recovery performance comparable to Agencourt AMPure® XP beads
  • For Research Use Only. Not for use in diagnostic procedures.

Storage Temperature
Store at 2-8 °C. Do NOT freeze.

Handling and Use To prevent contamination of the CleanMag Magnetic Beads, use sterile laboratory techniques when handling the product. Allow the product to equilibrate to room temperature before use and always vortex the solution vigorously prior to use to thoroughly resuspend the beads.

The volumetric ratio of the CleanMag Magnetic Beads solution to sample is critical to the size-selection performance. Ensure accurate aspiration and dispensing of the magnetic beads solution by pipetting slowly and wiping droplets of magnetic beads off the outside of the pipette tip.

CleanPlex NGS Panel User Guide

Menu